What sedimentary ancient DNA can tell us about past and future plant diversity?

Sedimentary ancient DNA reveals a direct correlation between plant taxa richness and alpine habitat in the south-eastern Tibetan Plateau since late glacial. Accordingly, plant taxa richness will decrease with warming-induced alpine habitat loss over 2050-2300 CE.

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Terrestrial plants, as primary producers, are the basis of ecosystem survival, which has observed and simulated upslope movement in high-mountain environments with an accelerated rate of climate warming.  Since the highest plant taxa richness is widely found in mid-elevation forest belts, upslope shift of forests is expected to increase the richness in upper elevational regions. However, how confident is this estimate based on short-term ecological data and species distribution modelling that hard to distinguish natural processes in many cases? “Maybe a long-term perspective answers”, my supervisor, Prof. Dr. Ulrike Herzschuh, suggested.

First of all, we selected an archive and set objectives. In February 2004, Steffen Mischke collected a 17.8 m sediment core from Lake Naleng in the south-eastern Tibetan Plateau, one of the world's biodiversity hotspots. This lake is a glacially-formed alpine lake at the upper treeline.

Landscape of south-eastern Tibetan Plateau (top-left) and Lake Naleng. Photo: Steffen Mischke and Richard H. Ree, private

Palaeoecological proxies from this core have documented the history of palaeoenvironment and palaeovegetation since the late glacial, such as glaciers dynamics, soil development, and shifts of treeline with temperature variations. In particular, such shifts may be partly linked to human activities that occurred in the late-Holocene. However, whether these variables drive plant diversity changes is still a question in this region. Furthermore, what the fate of plant diversity in high mountain regions in the context of climate changes in the following centuries? Does the plant diversity indeed increase along as mid-elevation forest belts rise? I was lucky to have an ideal archive from a perfect study site to investigate such exciting topics. Well begun is half done.

Then, we decided to use sedimentary ancient DNA (sedaDNA) to retrieve the plant taxa richness over the past ~18,000 years. SedaDNA is a powerful tool for site-specific palaeodiversity investigation compare to traditional proxies (pollen and macrofossil). However, no study focuses on high altitude regions in Tibetan Plateau. Our study could provide new insights into the causes of the plant taxa richness changes. I spent one year working at the genetic laboratory and analyzing the sedaDNA data. I was so excited to learn sedaDNA and relevant bioinformatics even though I faced a lot of challenges. Luckily, the extremely useful results were produced in collaboration with Heike H. Zimmermann, Kathleen R. Stoof-Leichsenring, and Laura S. Epp.

Due to the species-area relationship, past glacier dynamics and available habitat area with lake catchment should be the predictor variables. They were simulated by Dirk Scherler and Stefan Kruse, respectively. Moreover, plant survival is also supported by soil and temperature.  Thus, we collected the soil development record from the same lake sediment core temperature anomaly record based on multiple proxies in Northern Hemisphere (30°–90°N). We selected this temperature record because the climate at a millennial time scale on the eastern Tibetan Plateau is strongly influenced by East Asian summer monsoons that track changes in the mid- to high-latitude westerlies and continental warming.

Since one goal is understanding the effects of forest movement on plant taxa richness, we simulated the alpine habitat due to shifts of forest over the next 250 years in response to a predicted 2.5°C warming. Besides, modern plant species distribution in the south-eastern Tibetan Plateau, provided by Richard H. Ree, helps us interpret the spatial patterns of species occurrence.

SedaDNA records four vegetation stages: alpine steppe dominated 18–14 ka, alpine meadow 14–10 ka, open forest 10–3.6 ka, and alpine meadow after 3.6 ka with the presence of typical land-use indicators. Within these time intervals, total plant taxa richness was relatively low before 14 ka, higher during 14-10 ka, low again until 3.6 ka and high after 3.6 ka. We observed a similar pattern in within-family richness.

The figure illustrates the plant taxa richness (a-f), the predictor variables (g-j) and vegetation types (k) over the past ~ 18,000 years. Time intervals are determined by stratigraphically constrained clustering (l).

We correlated plant taxa richness with four predictor variables. We did not find evidence of a direct link between plant taxa richness and temperature. Instead, we found a contrasting relationship between them since 18 ka. The temperature may trigger different environmental processes that lead to such obvious contrast. Likewise, we did not find a statistically significant correlation between total plant taxa richness and glacier-related available habitat area, as well as soil development. Rapid glacier retreat had a negative impact before 14 ka.

The figure illustrates the correlation between total plant taxa richness and predictor variables across time-intervals.

We found a strong positive relationship between total plant taxa richness and forest-related alpine habitat after 14 ka. Although human impact promoted total plant taxa richness since 3.6 ka, its effect was smaller than that of alpine habitat. Consequently, a noticeable loss of alpine habitat with upslope advance of forest leads to a pronounced decrease in total plant taxa richness, especially for endemic-rich alpine plant families during 2050-2300 CE. With respect to conservation, alpine habitats should take priority. 

The figure illustrates the decline in total plant taxa richness induced by loss of alpine habitat in a warmer future.

Several shortcomings could introduce biases in plant taxa richness simulations. Nevertheless, this study is the first palaeo time-for-time approach that investigates the species-area relationship.  Accordingly, palaeoecological evidence can inform the biodiversity conservation in Tibetan.

This study spans over four years, almost my Ph.D. period. It is an excellent ending to my Ph.D. journey and also a new start in a part of my research career.

To get detail information, please read our paper: 10.1038/s41467-021-22986-4

 

Sisi Liu

PhD Candidate, Alfred Wegener Institute

I am interested in Paleoecology, working with ancient DNA and pollen from lake sediments from Tibetan Plateau and Northern Siberia.

Comments

Go to the profile of Gao Jianguo
4 months ago

Well done! Sisi. Glad to see you here.