A link to the paper in BMC Biology can be found here.
Also, we created a short demo video for the software:
Caenorhabditis elegans nematodes are powerful model organisms, yet quantification of visible phenotypes is still often labor-intensive, biased, and error-prone. Particularly, evolution research requires numerous samples, which make manual analysis extremely strenuous.
Hence, we developed WorMachine, a MATLAB-based image analysis software that utilized cutting-edge techniques from deep & machine learning, and consists of three steps:
- Automated identification of C. elegans worms in microscope images.
- Extraction of morphological features and quantification of fluorescent signals
- Machine learning techniques for high-level analysis.
We examined the power of WorMachine using five separate representative assays: supervised classification of binary-sex phenotype, scoring continuous-sexual phenotypes, quantifying the effects of two different RNA interference treatments, and measuring intracellular protein aggregation.
WorMachine is suitable for analysis of a variety of evolutionary questions and provides an accurate and reproducible analysis tool for measuring diverse phenotypes. It serves as a “quick and easy,” convenient, high-throughput, and automated solution for nematode research. We hope you find it helpful for your research needs, and are happy to be of service for any questions or comments.